Evaluation of visible diffuse reflectance spectroscopy in liver tissue: validation of tissue saturations using extracorporeal circulation

Significance: Real-time information about oxygen delivery to the hepatic graft is important direct care and diagnose vascular compromise in immediate post-transplant period. Aim: The current study was designed determine utility of visible diffuse reflectance spectroscopy (vis-DRS) for measuring liver tissue saturation vivo. Approach: A custom-built vis-DRS probe calibrated using phantoms with hemoglobin (Hb) polystyrene microspheres. Ex vivo (extracorporeal circulation) protocols were used a swine model (n = 15) validation via blood gas analysis. Results:In absorption scattering measured by without biliverdin correction correlated closely between analyses. Lin’s concordance correlation coefficients are 0.991 μa 0.959 μs ′ . Hb test (R2 0.81) 0.85) compared. Vis-DRS data obtained from ex protocol plotted against PO2 derived analysis showed good fit Hill coefficient 1.67 P50 34 mmHg 0.81). conversion formula developed account systematic deviation, which resulted goodness-of-fit R2 0.76 expected dissociation curve. Conclusions: We show that allows real-time measurement saturation, an indicator perfusion delivery.